Enzyme immunoassay of thyroxin-binding globulin.
نویسندگان
چکیده
منابع مشابه
Enzyme immunoassay of thyroxin-binding globulin.
We describe a new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum. A TBG-beta-D-galactosidase complex was prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester. The mean analytical recovery of TBG added to serum was 99%, sample volume and TBG value were linearly related, and values for TBG determined by this method and those determined by radioimmunoas...
متن کاملEnzyme immunoassay of thyroxin-binding globulin in dried blood samples on filter paper.
A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper ...
متن کاملEnzyme immunoassay of free thyroxin in serum.
We describe a double-antibody enzyme immunoassay for free thyroxin (FT4) in serum with use of beta-D-galactosidase conjugated to thyroxin. The method is uninfluenced by thyroxin-binding globulin or albumin. Values for FT4 so determined correlated well with those determined by radioimmunoassay (r = 0.98) and equilibrium dialysis (r = 0.89). The mean variability (CV) within and between assays was...
متن کاملNew one-step enzyme immunoassay for free thyroxin.
This enzyme immunoassay for free thyroxin (FT4) involves simultaneous incubation of sample and thyroxin conjugated to horseradish peroxidase (EC 1.11.1.7) in antibody-coated tubes at room temperature. The test can be performed in 90 min. It is not influenced by variations in concentrations of thyroxin-binding globulin (TBG), thyroxin-binding prealbumin, or albumin. With increased concentrations...
متن کاملFurther studies on thyroxin-binding globulin-dependence in equilibrium dialysis assays of free thyroxin.
is between 0.40 and 0.70 mmol/L. Granted, our patients are type I rather than type U diabetics, but both are allegedly prone to have increased concentrations of free glycerol in plasma. 3. We serve as the clinical laboratory for the University of Minnesota's hyperlipidemia clinic and I see patients in that clinic. This clinic's patient population is principally referral patients with more sever...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1982
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/28.12.2408